Anti-Cofilin 2 (phospho S3) antibody (ab14134)
Key features and details
- Rabbit polyclonal to Cofilin 2 (phospho S3)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Cofilin 2 (phospho S3) antibody
See all Cofilin 2 primary antibodies -
Description
Rabbit polyclonal to Cofilin 2 (phospho S3) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human Cofilin 2 aa 1-10 conjugated to keyhole limpet haemocyanin.
Sequence:MApSGVTVNDE-C
Database link: Q9Y281 -
Positive control
- WB: L6 whole cell lysate treated with LIM kinase.
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General notes
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cofilin 2 (phospho S3) antibody (ab14134)All lanes : Anti-Cofilin 2 (phospho S3) antibody (ab14134) at 4 µg/ml
Lane 1 : L6 lysate in RIPA buffer, untreated
Lane 2 : L6 lysate in RIPA buffer, treated with 100mU LIM Kinase 1 (active)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP)
Developed using the ECL technique.
Predicted band size: 19 kDaL6 lysate was incubated with or without 100 mU LIM Kinase 1 (active) at 30°C for 30 minutes. Phosphorylation was terminated by boiling in reducing sample buffer. Proteins were visualized using a chemiluminescence detection system.
