Anti-Clostridium difficile Toxin B antibody [EPR23357-19] - BSA and Azide free (ab270795)
![Anti-Clostridium difficile Toxin B antibody [EPR23357-19] - BSA and Azide free (ab270795)](https://www.abcam.com/ps/products/270/ab270795/Images/ab270795-384882-ANTICL1.JPG "Anti-Clostridium difficile Toxin B antibody [EPR23357-19] - BSA and Azide free (ab270795)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23357-19] to Clostridium difficile Toxin B - BSA and Azide free
- Suitable for: WB
- Reacts with: Clostridium difficile
Overview
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Product name
Anti-Clostridium difficile Toxin B antibody [EPR23357-19] - BSA and Azide free
See all Clostridium difficile Toxin B primary antibodies -
Description
Rabbit monoclonal [EPR23357-19] to Clostridium difficile Toxin B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Clostridium difficile -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Native Clostridium difficile Toxin B protein (ab124001) 10ng
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General notes
ab270795 is the carrier-free version of ab270452. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23357-19 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Clostridium difficile Toxin B antibody [EPR23357-19] - BSA and Azide free (ab270795)All lanes : Anti-Clostridium difficile Toxin B antibody [EPR23357-19] (ab270452) at 1/1000 dilution
Lane 1 : Native Clostridium difficile Toxin A protein (ab123999) 10ng
Lane 2 : Native Clostridium difficile Toxin B protein (ab124001) 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 270 kDa
Observed band size: 308 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Both the recombinant proteins were from Abcam.
Exposure time: lane 1: 3 minutes lane 2: 15 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270452).
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![Anti-Clostridium difficile Toxin B antibody [EPR23357-19] - BSA and Azide free (ab270795)](https://www.abcam.com/ps/products/270/ab270795/Images/ab270795-2-benefits-of-recombinant-antibodies.png)
Anti-Clostridium difficile Toxin B antibody [EPR23357-19] - BSA and Azide free (ab270795)