Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free (ab256474)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22840-25] to Cleaved Caspase-7 - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free
    See all Cleaved Caspase-7 primary antibodies

  • Description

    Rabbit monoclonal [EPR22840-25] to Cleaved Caspase-7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate, Wild-type HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate, mouse and rat spleen whole cell lysate. IP: HeLa treated with 1 µM Staurosporine for 3 hours whole cell lysate .

  • General notes

    ab256474 is the carrier-free version of ab256469. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256474 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free (ab256474)
    Western blot - Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free (ab256474)
    All lanes : Anti-Cleaved Caspase-7 antibody [EPR22840-25] (ab256469) at 1/1000 dilution

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate
    Lane 3 : Untreated wild-type HAP1 whole cell lysate
    Lane 4 : Wild-type HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
    Lane 5 : Untreated caspase-7 knockout HAP1 whole cell lysate
    Lane 6 : Caspase-7 knockout HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
    Lane 7 : Mouse spleen lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa
    Observed band size: 18,22 kDa
    why is the actual band size different from the predicted?



    Caspase-7 is activated upon apoptotic induction. Caspase-7 p22 subunit (22 kDa) and p20 subunit (18 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 16352606).  This antibody reacts with an unidentifiable protein around 48 kDa.  ab256469 was shown to specifically react with Caspase-7 in wild-type HAP1 cells as signal was lost in Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. ab256469 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ instrument using the ECL technique. Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256469).

  • Immunoprecipitation - Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free (ab256474)
    Immunoprecipitation - Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free (ab256474)

    Cleaved Caspase-7 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1µM Staurosporine for 3h whole cell lysate 10µg with ab256469 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256469. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1µM Staurosporine for 3h whole cell lysate 10µg

    Lane 2: ab256469 IP in HeLa treated with 1µM Staurosporine for 3h whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256469 in HeLa treated with 1µM Staurosporine for 3h whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    This antibody reacts with an unidentifiable protein around 48 kDa.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256469).

  • Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free (ab256474)
    Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free (ab256474)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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