Anti-CK1 epsilon antibody (ab264277)
Key features and details
- Rabbit polyclonal to CK1 epsilon
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CK1 epsilon antibody
See all CK1 epsilon primary antibodies -
Description
Rabbit polyclonal to CK1 epsilon -
Host species
Rabbit -
Specificity
This antibody may have slight crossreactivity with CKI delta, which has a similar epitope.
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Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human CK1 epsilon aa 366-416. The exact sequence is proprietary. NP_001885.1
Database link: P49674 -
Positive control
- IP: HeLa whole cell lysate; WB: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CK1 epsilon antibody (ab264277)All lanes : Anti-CK1 epsilon antibody (ab264277) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-CK1 epsilon antibody (ab264277)CK1 epsilon was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264277 at 10 μg per reaction. Western blot was performed on the immunoprecipitates using ab264277 at 1 μg/ml.
Lane 1: ab264277 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 3 seconds.
