Anti-CINAP antibody (ab240596)
Key features and details
- Rabbit polyclonal to CINAP
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CINAP antibody -
Description
Rabbit polyclonal to CINAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human CINAP aa 1-50. The exact sequence is proprietary.
Database link: Q9H2G4 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human testis and lung cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CINAP antibody (ab240596)All lanes : Anti-CINAP antibody (ab240596) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CINAP antibody (ab240596)Formalin-fixed, paraffin-embedded human testis tissue stained for CINAP using ab240596 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-CINAP antibody (ab240596)CINAP was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP; 20% of IP loaded) using ab240596 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab240596 at 1 μg/mL.
Lane 1: ab240596 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CINAP antibody (ab240596)Formalin-fixed, paraffin-embedded human lung cancer tissue stained for CINAP using ab240596 at 1/1000 dilution in immunohistochemical analysis. DAB staining.