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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Co-activators/co-repressors

Anti-CIA antibody (ab70831)

Anti-CIA antibody (ab70831)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to CIA
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CIA antibody
    See all CIA primary antibodies
  • Description

    Rabbit polyclonal to CIA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide within Human CIA aa 1-50 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Protein previously labeled as NCOA5.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70831 was affinity purified using an epitope specific to CIA immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIA antibody (ab70831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIA antibody (ab70831)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CIA with ab70831 at 1/200 (1µg/ml). Detection: DAB.

  • Western blot - Anti-CIA antibody (ab70831)
    Western blot - Anti-CIA antibody (ab70831)
    All lanes : Anti-CIA antibody (ab70831) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 3 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 66 kDa
    Observed band size: 66 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-CIA antibody (ab70831)
    Immunoprecipitation - Anti-CIA antibody (ab70831)

    Detection of CIA by Western Blot of Immunprecipitate.
    ab70831 at 1µg/ml staining CIA in HeLa whole cell lysate immunoprecipitated using ab70831 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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