Anti-Chd7 antibody (ab176807)
Key features and details
- Rabbit polyclonal to Chd7
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chd7 antibody
See all Chd7 primary antibodies -
Description
Rabbit polyclonal to Chd7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Chd7 aa 2947-2997. The exact sequence is proprietary. (NP_060250.2).
Sequence:FKDGETLEGSDAEESLDKTAESSLLEDEIAQGEELDSLDGGDEIENNEND E
Database link: Q9P2D1 -
Positive control
- HeLa whole cell lysate (ab150035).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab176807 was affinity purified using an epitope specific to Chd7 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Chd7 antibody (ab176807)All lanes : Anti-Chd7 antibody (ab176807) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 336 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-Chd7 antibody (ab176807)Detection of Chd7 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176807 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
