Anti-CHD1L antibody [2170C3a] (ab51324)
![Anti-CHD1L antibody [2170C3a] (ab51324)](https://www.abcam.com/ps/products/51/ab51324/Images/ab51324-63353-anti-chd1l-antibody-2170c3a-immunohistochemistry.jpg "Anti-CHD1L antibody [2170C3a] (ab51324)")
Key features and details
- Mouse monoclonal [2170C3a] to CHD1L
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CHD1L antibody [2170C3a]
See all CHD1L primary antibodies -
Description
Mouse monoclonal [2170C3a] to CHD1L -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human CHD1L fragment
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Positive control
- HEK293 whole cell lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
2170C3a -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHD1L antibody [2170C3a] (ab51324)IHC image of ab51324 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51324, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-CHD1L antibody [2170C3a] (ab51324)](https://www.abcam.com/ps/products/51/ab51324/Images/ab51324-63355-anti-chd1l-antibody-2170c3a-western-blot.gif)
Western blot - Anti-CHD1L antibody [2170C3a] (ab51324)Anti-CHD1L antibody [2170C3a] (ab51324) at 1/50 dilution + HEK293 whole cell lysate at 25 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 101 kDa
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![Immunoprecipitation - Anti-CHD1L antibody [2170C3a] (ab51324)](https://www.abcam.com/ps/products/51/ab51324/Images/ab51324-63354-anti-chd1l-antibody-2170c3a-immunoprecipitation.gif)
Immunoprecipitation - Anti-CHD1L antibody [2170C3a] (ab51324)Immunoprecipitation analysis using ab51324 at 5ug/500ug RIPA lysate of HeLa cells. Immunoblot uisng ab51324 at 1/50 dilution and 1/2500 anti-mouse IgG as secondary.
