Anti-Chd1 antibody (ab242098)
Key features and details
- Rabbit polyclonal to Chd1
- Suitable for: ChIP/Chip, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Chd1 antibody
See all Chd1 primary antibodies -
Description
Rabbit polyclonal to Chd1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP/Chip HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human Chd1 aa 1660-1710. The exact sequence is proprietary.
Database link: O14646 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. ChIP on ChIP: K562 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Chd1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Chd1 antibody (ab242098) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 seconds
-
Chd1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242098 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab242098 at 1 µg/ml.
Lane 1: ab242098 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
-
ab242098 (10 μg) was used to immunoprecipitate chromatin from K562 cells according to the protocol of Ren et al (Genes Dev. 2002 16: 245-256).
(A) Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites.
(B) As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Chd1 ChIP, normal rabbit IgG showed little enrichment.