Anti-Cenexin1/ODF2 antibody (ab43840)
Key features and details
- Rabbit polyclonal to Cenexin1/ODF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cenexin1/ODF2 antibody
See all Cenexin1/ODF2 primary antibodies -
Description
Rabbit polyclonal to Cenexin1/ODF2 -
Host species
Rabbit -
Specificity
On the basis of the immunogen sequence, we would expect this antibody to recognise the following isoforms of Cenexin1/ODF2 as listed in Swissprot: Q5BJF6-1 (95 kDa), Q5BJF6-2 (89 kDa), Q5BJF6-3 (93 kDa), and Q5BJF6-4 (95 kDa). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human Cenexin1/ODF2 aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab43839)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab43840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Target
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Function
Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly. -
Tissue specificity
Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails. -
Sequence similarities
Belongs to the ODF2 family. -
Post-translational
modificationsTyrosine phosphorylated. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles. - Information by UniProt
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Database links
- Entrez Gene: 4957 Human
- Entrez Gene: 18286 Mouse
- Omim: 602015 Human
- SwissProt: Q5BJF6 Human
- SwissProt: A3KGV1 Mouse
- Unigene: 129055 Human
- Unigene: 330116 Mouse
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Alternative names
- 84 kDa outer dense fiber protein antibody
- Cancer/testis antigen 134 antibody
- Cenexin 1 antibody
see all
Images
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Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)All lanes : ab43840 at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 25 µg
Lane 2 : MCF7 (Human breast adenocarcinoma) at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
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Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (ab43840)This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.Immunocytochemistry/ Immunofluorescence analysis of human HCT116 cells labeling Cenexin1/ODF2 with ab43840 at 1/400 dilution. Cells were fixed in formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab43840 at 1/400 was carried out for 1 hour at 22°C in PBS buffer. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.
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Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)Image courtesy of an anonymous abreview.All lanes : Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution
Lane 1 : 25 ug HeLa cell lysate
Lane 2 : 12.5 ug HeLa cell lysate
Lane 3 : 25 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
Lane 4 : 12.5 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
Secondary
All lanes : Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: ~75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.
Protocols
Datasheets and documents
References (22)
ab43840 has been referenced in 22 publications.
- Chong WM et al. Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages. Elife 9:N/A (2020). PubMed: 32242819
- Kashihara H et al. Cep128 associates with Odf2 to form the subdistal appendage of the centriole. Genes Cells 24:231-243 (2019). PubMed: 30623524
- Watanabe K et al. The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Nat Commun 10:931 (2019). PubMed: 30804344
- Lecland N et al. Epidermal development requires ninein for spindle orientation and cortical microtubule organization. Life Sci Alliance 2:N/A (2019). PubMed: 30923192
- Tsai JJ et al. CEP120 interacts with C2CD3 and Talpid3 and is required for centriole appendage assembly and ciliogenesis. Sci Rep 9:6037 (2019). PubMed: 30988386
Images
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Western blot - Anti-Cenexin1/ODF2 antibody (ab43840)All lanes : ab43840 at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 25 µg
Lane 2 : MCF7 (Human breast adenocarcinoma) at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesAbcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
-
Immunocytochemistry/ Immunofluorescence - Anti-Cenexin1/ODF2 antibody (ab43840) This image is courtesy of an Abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB.
Immunocytochemistry/ Immunofluorescence analysis of human HCT116 cells labeling Cenexin1/ODF2 with ab43840 at 1/400 dilution. Cells were fixed in formaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Staining with ab43840 at 1/400 was carried out for 1 hour at 22°C in PBS buffer. ab150081, a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody was used at 1/200 dilution. DAPI was used to counterstain.
-
Western blot - Anti-Cenexin1/ODF2 antibody (ab43840) Image courtesy of an anonymous abreview.All lanes : Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution
Lane 1 : 25 ug HeLa cell lysate
Lane 2 : 12.5 ug HeLa cell lysate
Lane 3 : 25 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
Lane 4 : 12.5 ug HeLa cell lysate with Human Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
Secondary
All lanes : Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: ~75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.
