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Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14793(B)] to CECR5 - C-terminal
  • Suitable for: WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CECR5 antibody [EPR14793(B)] - C-terminal
    See all CECR5 primary antibodies

  • Description

    Rabbit monoclonal [EPR14793(B)] to CECR5 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, HeLa, Jurkat, HepG2, U87-MG cell lysates. ICC: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14793(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    Western blot - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : CECR5 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab184185 observed at 46 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab184185 Anti-CECR5 antibody [EPR14793(B)] - C-terminal was shown to specifically react with CECR5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266708 (knockout cell lysate ab257888) was used. Wild-type and CECR5 knockout samples were subjected to SDS-PAGE. ab184185 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    Immunocytochemistry/ Immunofluorescence - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)

    Immunofluorescent analysis of HeLa cells labeling CECR5 with ab184185 at a 1/100 dilution. Primary incubation was followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • Western blot - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    Western blot - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa

  • Western blot - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    Western blot - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    All lanes : Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 46 kDa

  • Immunoprecipitation - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    Immunoprecipitation - Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling CECR5 with ab184185. Right lane shows PBS negative control.

  • Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)
    Anti-CECR5 antibody [EPR14793(B)] - C-terminal (ab184185)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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