Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)
![Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)](https://www.abcam.com/ps/products/254/ab254214/Images/ab254214-2-253238IP2.jpg "Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22334-99] to CDNF - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CDNF antibody [EPR22334-99] - BSA and Azide free
See all CDNF primary antibodies -
Description
Rabbit monoclonal [EPR22334-99] to CDNF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Human skeletal muscle tissue and testis lysate.
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General notes
Ab254214 is the carrier-free version of ab253238. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab254214 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22334-99 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)
CDNF was immunoprecipitated from 0.35 mg human testis tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.
Lane 1: Human testis tissue lysate 10 µg (input).
Lane 2: ab253238 IP in human testis tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human testis tissue lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253238).
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![Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)](https://www.abcam.com/ps/products/254/ab254214/Images/ab254214-1-253238IP1.jpg)
Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)CDNF was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.
Lane 1: Human skeletal muscle tissue lysate 10 µg (input).
Lane 2: ab253238 IP in human skeletal muscle tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human skeletal muscle lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253238).
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![Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)](https://www.abcam.com/ps/products/254/ab254214/Images/ab254214-3-benefits-of-recombinant-antibodies.png)
Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)
