Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22334-99] to CDNF - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CDNF antibody [EPR22334-99] - BSA and Azide free
    See all CDNF primary antibodies

  • Description

    Rabbit monoclonal [EPR22334-99] to CDNF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Human skeletal muscle tissue and testis lysate.

  • General notes

    Ab254214 is the carrier-free version of ab253238. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254214 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)
    Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)

    CDNF was immunoprecipitated from 0.35 mg human testis tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

    Lane 1: Human testis tissue lysate 10 µg (input).
    Lane 2: ab253238 IP in human testis tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human testis tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253238).

  • Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)
    Immunoprecipitation - Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)

    CDNF was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.

    Lane 1: Human skeletal muscle tissue lysate 10 µg (input).
    Lane 2: ab253238 IP in human skeletal muscle tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human skeletal muscle lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253238).

  • Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)
    Anti-CDNF antibody [EPR22334-99] - BSA and Azide free (ab254214)

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