Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

500 µl 311 587 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Mouse monoclonal [C68/684] to CD68
Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-CD68 antibody [C68/684]
See all CD68 primary antibodies
Description

Mouse monoclonal [C68/684] to CD68
Host species

Mouse
Specificity

ab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).
Positive control
- IHC-P: Human tonsil and histiocytoma tissues; ICC/IF: U-87 MG cells; Flow Cyt: U-87 MG cells; WB: Human spleen tissue lysate.
General notes

ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

ab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography.
Clonality

Monoclonal
Clone number

C68/684
Isotype

IgG1
Light chain type

kappa
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [C68/684] (ab201340)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.
Flow Cytometry - Anti-CD68 antibody [C68/684] (ab201340)

Flow Cytometry analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (blue); Isotype Control (red).
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [C68/684] (ab201340)

Immunocytochemistry/immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labelling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (green). Nuclear DNA stained red.
Western blot - Anti-CD68 antibody [C68/684] (ab201340)

Anti-CD68 antibody [C68/684] (ab201340) + Human spleen tissue lysate

Predicted band size: 37 kDa
Additional bands at: 110 kDa (possible glycosylated form)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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