Anti-CD68 antibody [3F7D3] (ab201973)
![Anti-CD68 antibody [3F7D3] (ab201973)](https://www.abcam.com/ps/products/201/ab201973/Images/ab201973-243292-anti-cd68-antibody-3f7d3-immunohistochemistry.jpg "Anti-CD68 antibody [3F7D3] (ab201973)")
Key features and details
- Mouse monoclonal [3F7D3] to CD68
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD68 antibody [3F7D3]
See all CD68 primary antibodies -
Description
Mouse monoclonal [3F7D3] to CD68 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CD68 aa 1-200. (Purified from E.coli).
Database link: P34810 -
Positive control
- Human CD68 (aa42-155) recombinant protein; CD68 (aa42-155)-hIgGFc transfected HEK293 cell lysate; HeLa cells; U937, HeLa, HepG2 and Jurkat whole cell lysate (ab7899); Human endometrial cancer and bladder cancer tissues.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR255595-19 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3F7D3 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [3F7D3] (ab201973)
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD68 with ab201973 at 1/200 dilution followed by DAB staining.
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![Western blot - Anti-CD68 antibody [3F7D3] (ab201973)](https://www.abcam.com/ps/products/201/ab201973/Images/ab201973-243297-anti-cd68-antibody-3f7d3-western-blot.jpg)
Western blot - Anti-CD68 antibody [3F7D3] (ab201973)Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution + Human CD68 (aa42-155) recombinant protein
Predicted band size: 37 kDaExpected MWt is 37.4 kDa.
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![Western blot - Anti-CD68 antibody [3F7D3] (ab201973)](https://www.abcam.com/ps/products/201/ab201973/Images/ab201973-243296-anti-cd68-antibody-3f7d3-western-blot.jpg)
Western blot - Anti-CD68 antibody [3F7D3] (ab201973)All lanes : Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : CD68 (aa42-155)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 37 kDa
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![Western blot - Anti-CD68 antibody [3F7D3] (ab201973)](https://www.abcam.com/ps/products/201/ab201973/Images/ab201973-243295-ab201973WBc.jpg)
Western blot - Anti-CD68 antibody [3F7D3] (ab201973)All lanes : Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution
Lane 1 : U937 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Predicted band size: 37 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [3F7D3] (ab201973)](https://www.abcam.com/ps/products/201/ab201973/Images/ab201973-243294-anti-cd68-antibody-3f7d3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [3F7D3] (ab201973)Immunofluorescent analysis of HeLa cells labeling CD68 with ab201973 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
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![Flow Cytometry - Anti-CD68 antibody [3F7D3] (ab201973)](https://www.abcam.com/ps/products/201/ab201973/Images/ab201973-243293-anti-cd68-antibody-3f7d3-flow-cytometry.jpg)
Flow Cytometry - Anti-CD68 antibody [3F7D3] (ab201973)Flow cytometric analysis of HeLa cells labeling CD68 with ab201973 at 1/200 dilution (green) compared to a negative control (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [3F7D3] (ab201973)](https://www.abcam.com/ps/products/201/ab201973/Images/ab201973-243291-anti-cd68-antibody-3f7d3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [3F7D3] (ab201973)Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling CD68 with ab201973 at 1/200 dilution followed by DAB staining.
