Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µg 274 732 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [3F7D3] to CD68
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Human
Isotype: IgG1

Product name

Anti-CD68 antibody [3F7D3]
See all CD68 primary antibodies
Description

Mouse monoclonal [3F7D3] to CD68
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD68 aa 1-200. (Purified from E.coli).
Database link: P34810

Run BLAST with Run BLAST with
Positive control
- Human CD68 (aa42-155) recombinant protein; CD68 (aa42-155)-hIgGFc transfected HEK293 cell lysate; HeLa cells; U937, HeLa, HepG2 and Jurkat whole cell lysate (ab7899); Human endometrial cancer and bladder cancer tissues.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR255595-19 are from Tissue Culture Supernatant

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

3F7D3
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [3F7D3] (ab201973)

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD68 with ab201973 at 1/200 dilution followed by DAB staining.
Western blot - Anti-CD68 antibody [3F7D3] (ab201973)

Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution + Human CD68 (aa42-155) recombinant protein

Predicted band size: 37 kDa

Expected MWt is 37.4 kDa.
Western blot - Anti-CD68 antibody [3F7D3] (ab201973)

All lanes : Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : CD68 (aa42-155)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 37 kDa
Western blot - Anti-CD68 antibody [3F7D3] (ab201973)

All lanes : Anti-CD68 antibody [3F7D3] (ab201973) at 1/500 dilution

Lane 1 : U937 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate

Predicted band size: 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [3F7D3] (ab201973)

Immunofluorescent analysis of HeLa cells labeling CD68 with ab201973 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry - Anti-CD68 antibody [3F7D3] (ab201973)

Flow cytometric analysis of HeLa cells labeling CD68 with ab201973 at 1/200 dilution (green) compared to a negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [3F7D3] (ab201973)

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling CD68 with ab201973 at 1/200 dilution followed by DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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