Anti-CD45RA antibody [MRC OX-33] - BSA and Azide free (ab244569)
![Anti-CD45RA antibody [MRC OX-33] - BSA and Azide free (ab244569)](https://www.abcam.com/ps/products/244/ab244569/Images/ab244569-351123-anti-CD45RA-antibody-mrc-ox-33-flowcytometryratsplenocytes.jpg "Anti-CD45RA antibody [MRC OX-33] - BSA and Azide free (ab244569)")
Key features and details
- Mouse monoclonal [MRC OX-33] to CD45RA - BSA and Azide free
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CD45RA antibody [MRC OX-33] - BSA and Azide free
See all CD45RA primary antibodies -
Description
Mouse monoclonal [MRC OX-33] to CD45RA - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length native protein (purified) corresponding to Rat CD45RA. Purified from spleen.
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Positive control
- Flow Cyt: Lewis rat splenocytes. IHC-Fr: Rat Spleen
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General notes
ab244569 is a PBS only version of ab33933.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line. This clone has been described reacting with paraffin embedded material following PLP fixation (see Whiteland et al., 1995). It only labels B cells among thoracic duct lymphocytes, with little labelling in bone marrow and none on thymocytes (Barclay et al., 1987).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
MRC OX-33 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD45RA antibody [MRC OX-33] - BSA and Azide free (ab244569)
Lewis rat splenocytes stained with ab33933 (right) or mouse IgG1κ (ab170190) isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33933) or mouse IgG1κ isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33933).
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![Immunohistochemistry (Frozen sections) - Anti-CD45RA antibody [MRC OX-33] - BSA and Azide free (ab244569)](https://www.abcam.com/ps/products/244/ab244569/Images/ab244569-365882-anticd45ramrcox33ihcfrratspleen.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD45RA antibody [MRC OX-33] - BSA and Azide free (ab244569)This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33933).
IHC image of CD45RA staining in a section of frozen normal Rat Spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab33933 at 1µg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue). The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
