Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E75] to CCR7 - BSA and Azide free
  • Suitable for: ELISA, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CCR7 antibody [E75] - BSA and Azide free
    See all CCR7 primary antibodies

  • Description

    Rabbit monoclonal [E75] to CCR7 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises CCR7/CKR7. It does not cross react with other CKR family members.

  • Tested applications

    Suitable for: ELISA, WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247223 is the carrier-free version of ab32075. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247223 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
    Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
    All lanes : Anti-CCR7 antibody [E75] (ab32075) at 1/1000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 2 : Human lung lysate
    Lane 3 : Human lymph node lysate
    Lane 4 : Human spleen lysate
    Lane 5 : Human plasma lysate
    Lane 6 : Human brain lysate (low/negative expression)
    Lane 7 : Human liver lysate (low/negative expression)
    Lane 8 : Mouse lung lysate
    Lane 9 : Mouse lymph node lysate
    Lane 10 : Mouse spleen lysate
    Lane 11 : Mouse plasma lysate
    Lane 12 : Rat lung lysate
    Lane 13 : Rat lymph node tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32075, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
    Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
    Anti-CCR7 antibody [E75] (ab32075) at 1/500 dilution + Daudi cell lysate

    Predicted band size: 43 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    This data was developed using ab32075, the same antibody clone in a different buffer formulation.

     

  • ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
    ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)

    This data was developed using ab32075, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human CCR7 recombinant protein at 250 ng/mL with ab32075. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
    Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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