Anti-CCN3 antibody (ab137677)
Key features and details
- Rabbit polyclonal to CCN3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CCN3 antibody
See all CCN3 primary antibodies -
Description
Rabbit polyclonal to CCN3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 58-357 of Human CCN3 (Uniprot ID: P48745).
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Positive control
- Recombinant Human CCN3 protein (ab50076) can be used as a positive control in WB. 293T, A431, H1299, HeLaS2, Hela, HepG2, MOLT4, Mouse brain and Raji whole cell lysates; HeLa cells; Human gastric carcinoma N87 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-CCN3 antibody (ab137677) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 39 kDa
12% SDS PAGE -
Anti-CCN3 antibody (ab137677) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 39 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling CCN3 using ab137677 at 1/200 dilution. Lower image is merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma N87 xenograft tissue, labelling CCN3 with ab137677 at 1/100 dilution.