Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23610 -40] to CCL4/MIP-1 beta - BSA and Azide free
  • Suitable for: Flow Cyt, Dot blot, ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free
    See all CCL4/MIP-1 beta primary antibodies

  • Description

    Rabbit monoclonal [EPR23610 -40] to CCL4/MIP-1 beta - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody has cross-reactivity with CCL4L1. There is about 97% homology between CCL4/MIP-1 beta and CCL4L1 in terms of the immunogen designed from CCL4/MIP-1 beta.

  • Tested applications

    Suitable for: Flow Cyt, Dot blot, ICCmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: THP-1 cells (treated with PMA, 100nM for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 µg/ml) for another 3 hours.). Flow cyt: THP-1 (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) and RAW 264.7 cells ((reated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours).

  • General notes

    ab275399 is the carrier-free version of ab254371. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)
    Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)

    This data was developed using ab254371, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100nM phorbol 12-myristate 13-acetate (PMA) for 16 hours, then 100 ng/ml lipopolysaccharide (LPS) for 4 hours and add 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red)/Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/500 dilution (0.1ug)/ compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    The expression pattern is consistent with what is described in the literature (PMID:29669317).

  • Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)
    Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)

    This data was developed using ab254371, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCL4/MIP-1 beta with ab254371 at 1/100 (5.45 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cells treated with phorbol 12-myristate 13-acetate (PMA, 100nM) for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 ug/ml) for another 3 hours. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)
    Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)

    This data was developed using ab254371, the same antibody clone in a different buffer formulation.

    Dot blot analysis of CCL4/MIP-1 beta using ab254371 at 1/1000 (0.545ug/ml) dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution.

    Lane 1: Human CCL4/MIP-1 beta immunogen recombinant protein

    Lane 2: Human CCL4L1 recombinant protein

    Lane 3:  Bovine serum albumin (BSA)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 114 seconds

  • Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)
    Flow Cytometry - Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)

    This data was developed using ab254371, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red) /Untreated control (Green) cells labelling CCL4/MIP-1 beta with ab254371 at 1/50 dilution (1ug)/ compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    The expression pattern is consistent with what is described in the literature (PMID:32230927).

  • Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)
    Anti-CCL4/MIP-1 beta antibody [EPR23610 -40] - BSA and Azide free (ab275399)

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