Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP521Y] to CCL4/MIP-1 beta - BSA and Azide free
  • Suitable for: ELISA, ICC, IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free
    See all CCL4/MIP-1 beta primary antibodies

  • Description

    Rabbit monoclonal [EP521Y] to CCL4/MIP-1 beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab187674 is the carrier-free version of ab45690 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab187674 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Macrophage Inflammatory Protein 1 beta, MIP1 beta

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    Western blot - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    All lanes : Anti-CCL4/MIP-1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untagged human CCL4 recombinant protein (aa24-92)
    Lane 2 : Untagged human CCL4L recombinant protein (aa24-92)
    Lane 3 : GST-tagged human CCL3 recombinant protein (aa27-92)
    Lane 4 : GST-tagged human CCL3L recombinant protein 2*(aa28-93)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab45690, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    Immunocytochemistry - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    This data was developed using ab45690, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) cells labeling CCL4/MIP-1 beta + CCL4L with ab45690 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. DAPI was used to stain nuclei blue. The expression increased after treatment with Lipopolysaccharides (LPS), 100 ng/mL for 4 hours, followed by addition of Brefeldin A (1 μg/mL) for 3 hours.
  • Western blot - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    Western blot - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    All lanes : Anti-CCL4/MIP-1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human acute monocytic leukemia) cell lysate
    Lane 2 : THP-1 treated with 100 nM Phorbol-12-myristate-13-acetate(PMA) overnight, then treated with Lipopolysaccharides (LPS) 100 ng/mL for 7 hours and then 1 µg/mL Brefeldin A was added for the last 3 hours, lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 10 kDa
    Observed band size: 12 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab45690, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    CCL4/MIP-1 beta is induced in macrophages following exposure to bacterial LPS (PMID: 9848081).

  • Immunoprecipitation - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    Immunoprecipitation - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)

    This data was developed using ab45690, the same antibody clone in a different buffer formulation.

    ab45690 at 1/60 immunoprecipitating CCL4/MIP-1 beta + CCL4L in THP-1 (Human monocytic leukemia cell line) whole cell lysate observed at 12 KDa (lanes 1 and 2).

    Lane 1 (input): THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 µg/mL Brefeldin A was added for the last 3 hours whole cell lysate, 10µg.

    Lane 2 (+): ab45690 + THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 µg/mL Brefeldin A was added for the last 3 hours whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45690 in THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 µg/mL Brefeldin A was added for the last 3 hours whole cell lysate.

    For western blotting, ab45690 at 1/1000 and ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/1000).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    Western blot - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    All lanes : Anti-CCL4/MIP-1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untreated Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
    Lane 2 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) treated with LPS 10µg/mL for 4 hours and then 1 µg/mL Brefeldin A was added for the last 3 hours lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 10 kDa


    Exposure time: 3 minutes


    This data was developed using ab45690, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    ELISA - Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)

    This data was developed using ab45690, the same antibody clone in a different buffer formulation.

    ELISA analysis of Human CCL4/MIP-1 beta recombinant protein at 500 ng/mL with ab45690. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)
    Anti-CCL4/MIP-1 beta antibody [EP521Y] - BSA and Azide free (ab187674)

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