Anti-CBFb antibody [EPR6321] - BSA and Azide free (ab247963)
![Anti-CBFb antibody [EPR6321] - BSA and Azide free (ab247963)](https://www.abcam.com/ps/products/247/ab247963/Images/ab247963-397386-anti-cbfb-antibody-epr6321-western-blot-a431-wt-a431-cbfb-knockout-k562-hela.jpg "Anti-CBFb antibody [EPR6321] - BSA and Azide free (ab247963)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6321] to CBFb - BSA and Azide free
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-CBFb antibody [EPR6321] - BSA and Azide free
See all CBFb primary antibodies -
Description
Rabbit monoclonal [EPR6321] to CBFb - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, K562 and HeLa cell lysates.
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General notes
ab247963 is the carrier-free version of ab124693 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247963 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.97 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6321 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CBFb antibody [EPR6321] - BSA and Azide free (ab247963)All lanes : Anti-CBFb antibody [EPR6321] (ab124693) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : CBFB knockout A431 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 24-25 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab124693).
Lanes 1 - 4: Merged signal (red and green). Green - ab124693 observed at 24-25 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab124693 was shown to react with CBFb in wild-type A431 cells in western blot. Loss of signal was observed when CBFB knockout sample was used. Wild-type and CBFB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124693 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-CBFb antibody [EPR6321] - BSA and Azide free (ab247963)](https://www.abcam.com/ps/products/247/ab247963/Images/ab247963-2-benefits-of-recombinant-antibodies.png)
Anti-CBFb antibody [EPR6321] - BSA and Azide free (ab247963)