Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18108] to Caspase-9 - BSA and Azide free
  • Suitable for: IP, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free
    See all Caspase-9 primary antibodies

  • Description

    Rabbit monoclonal [EPR18108] to Caspase-9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250848 is the carrier-free version of ab185719. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250848 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)

    This data was developed using ab185719, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Caspase-9 knockout HAP1 cell lysate (20 µg) 

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: Jurkat cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab185719 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab185719 was shown to specifically react with Caspase-9 when Caspase-9 knockout samples were used. Wild-type and Caspase-9 knockout samples were subjected to SDS-PAGE. ab185719 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/10000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa whole cell lysate treated with staurosporine 1uM for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 35,37,46 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab185719, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/2500 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
    Lane 2 : NIH/3T3 whole cell lysate treated with staurosporine 1uM for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 37,39,49 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab185719, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 30 seconds


    This data was developed using ab185719, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/5000 dilution

    Lane 1 : Human pancreas tissue lysate
    Lane 2 : Human fetal liver tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa


    Exposure time: 30 seconds


    This data was developed using ab185719, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    Western blot - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    All lanes : Anti-Caspase-9 antibody [EPR18108] (ab185719) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 46 kDa
    Observed band size: 37,39,49 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab185719, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    Immunoprecipitation - Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    This data was developed using ab185719, the same antibody clone in a different buffer formulation.Caspase-9 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with staurosporine 1uM for 4 hours with ab185719 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab185719 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HeLa whole cell lysate treated with staurosporine 10 µg (Input).Lane 2: ab185719 IP in HeLa whole cell lysate treated with staurosporine.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185719 in HeLa whole cell lysate treated with staurosporine.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  • Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)
    Anti-Caspase-9 antibody [EPR18108] - BSA and Azide free (ab250848)

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