Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free
    See all Caspase-6/CASP-6 primary antibodies

  • Description

    Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects both the full length and cleaved forms of Caspase-6/CASP-6.

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247635 is the carrier-free version of ab108335. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247635 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CASP6 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 33 kDa



    This data was developed using ab108335, the same antibody clone in a different buffer formulation.

    Lanes 1 - 2: Merged signal (red and green). Green - ab108335 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.

    ab108335 was shown to specifically react with in wild-type HAP1 cells as signal was lost in CASP6 knockout cells. Wild-type and CASP6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab108335 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat + staurosporine cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 33 kDa



    This data was developed using ab108335, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
    Flow Cytometry - Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
    This data was developed using ab108335, the same antibody clone in a different buffer formulation.ab108335, at 1:10 dilution, staining Caspase-6/CASP-6 in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG shown in green (negative).
  • Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)
    Anti-Caspase-6/CASP-6 antibody [EPR4405] - BSA and Azide free (ab247635)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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