Anti-CaMKII antibody [6G9] (ab22609)
![Anti-CaMKII antibody [6G9] (ab22609)](https://www.abcam.com/ps/products/22/ab22609/Images/ab22609-32376-anti-camkii-antibody-6g9-western-blot.jpg "Anti-CaMKII antibody [6G9] (ab22609)")
Key features and details
- Mouse monoclonal [6G9] to CaMKII
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CaMKII antibody [6G9]
See all CaMKII primary antibodies -
Description
Mouse monoclonal [6G9] to CaMKII -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Rat
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Immunogen
Full length native protein (purified) corresponding to Rat CaMKII.
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Positive control
- WB: Human, rat, and mouse whole brain tissue lysates; IHC-P: Rat normal brain FFPE tissue sections.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
6G9 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-CaMKII antibody [6G9] (ab22609)All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 65 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [6G9] (ab22609)](https://www.abcam.com/ps/products/22/ab22609/Images/ab22609-341597-ap4529156assy72277jpgdatasheet.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII antibody [6G9] (ab22609)IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
