Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564)
![Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564)](https://www.abcam.com/ps/products/191/ab191564/Images/ab191564-229126-ab191564WB1.jpg "Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15227(B)] to Calsequestrin 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Calsequestrin 1 antibody [EPR15227(B)]
See all Calsequestrin 1 primary antibodies -
Description
Rabbit monoclonal [EPR15227(B)] to Calsequestrin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatHumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human, mouse and rat skeletal muscle lysates; Human and rat skeletal muscle tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15227(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)](https://www.abcam.com/ps/products/191/ab191564/Images/ab191564-229125-ab191564WB2.jpg)
Western blot - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)All lanes : Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564) at 1/10000 dilution
Lane 1 : mouse muscle lysate
Lane 2 : rat muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)](https://www.abcam.com/ps/products/191/ab191564/Images/ab191564-229124-ab191564IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)Immunohistochemical analysis of paraffin-embedded, Human skeletal muscle tissue labeling Calsequestrin 1 with ab191564 at a 1/250 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)](https://www.abcam.com/ps/products/191/ab191564/Images/ab191564-229123-ab191564IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)Immunohistochemical analysis of paraffin-embedded, rat skeletal muscle tissue labeling Calsequestrin 1 with ab191564 at a 1/250 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564)](https://www.abcam.com/ps/products/191/ab191564/Images/ab191564-5-benefits-of-recombinant-antibodies.png)
Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564)
