Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15227(B)] to Calsequestrin 1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Calsequestrin 1 antibody [EPR15227(B)]
    See all Calsequestrin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15227(B)] to Calsequestrin 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human, mouse and rat skeletal muscle lysates; Human and rat skeletal muscle tissues.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15227(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)
    Western blot - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)
    Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 63 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)
    Western blot - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)
    All lanes : Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564) at 1/10000 dilution

    Lane 1 : mouse muscle lysate
    Lane 2 : rat muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 63 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)

    Immunohistochemical analysis of paraffin-embedded, Human skeletal muscle tissue labeling Calsequestrin 1 with ab191564 at a 1/250 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 1 [EPR15227(B)] antibody (ab191564)

    Immunohistochemical analysis of paraffin-embedded, rat skeletal muscle tissue labeling Calsequestrin 1 with ab191564 at a 1/250 dilution. Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564)
    Anti-Calsequestrin 1 antibody [EPR15227(B)] (ab191564)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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