Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP710Y] to CAD/BM1 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free
    See all CAD/BM1 primary antibodies

  • Description

    Rabbit monoclonal [EP710Y] to CAD/BM1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is specific to the N-terminus of CAD/BM1.

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab231694 is the carrier-free version of ab40800 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab231694 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as CAD

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Western blot - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Anti-CAD/BM1 antibody [EP710Y] (ab40800) at 1/2000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 243 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab40800, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Immunocytochemistry - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)

    This data was developed using ab40800, the same antibody clone in a different buffer formulation.

    Immunofluorescence staining of HeLa cells with purified ab40800 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab40800 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
  • Flow Cytometry - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Flow Cytometry - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)

    This data was developed using ab40800, the same antibody clone in a different buffer formulation.

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CAD/BM1 (red) with ab40800 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
  • Immunoprecipitation - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Immunoprecipitation - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    This data was developed using ab40800, the same antibody clone in a different buffer formulation.ab40800 (purified) at 1/30 immunoprecipitating CAD/BM1 in 10 μg HeLa (Lanes 1 and 2, observed at 250 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Western blot - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Western blot - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Anti-CAD/BM1 antibody [EP710Y] (ab40800) at 1/1000 dilution (purified) + HeLa cell lysate at 1/20000 dilution

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 243 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    This data was developed using ab40800, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Immunocytochemistry - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    This data was developed using ab40800, the same antibody clone in a different buffer formulation.Unpurified ab40800 (1/100), staining human CAD/BM1 (N-term) in HeLa cells by Immunofluorescence.
  • Western blot - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Western blot - Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Anti-CAD/BM1 antibody [EP710Y] (ab40800) at 1/1000 dilution (unpurified)
    Predicted band size: 243 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    This data was developed using ab40800, the same antibody clone in a different buffer formulation.

  • Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)
    Anti-CAD/BM1 antibody [EP710Y] - BSA and Azide free (ab231694)

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