Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CacyBP antibody [EPR12374] (ab171972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12374] to CacyBP
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CacyBP antibody [EPR12374]
    See all CacyBP primary antibodies

  • Description

    Rabbit monoclonal [EPR12374] to CacyBP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human CacyBP aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9HB71

  • Positive control

    • Human kidney, gastric carcinoma and gliocytoma tissues; MCF7, HeLa, 293T and PC3 cell lysates; MCF7 and 293T cells.

  • General notes

     This product was previously labelled as SIAH Interacting Protein

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12374

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CacyBP antibody [EPR12374] (ab171972)
    Western blot - Anti-CacyBP antibody [EPR12374] (ab171972)
    All lanes : Anti-CacyBP antibody [EPR12374] (ab171972) at 1/10000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : PC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)

    Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling CacyBP with ab171972 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)

    Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CacyBP antibody [EPR12374] (ab171972)
    Immunocytochemistry/ Immunofluorescence - Anti-CacyBP antibody [EPR12374] (ab171972)

    Immunofluorescent analysis of MCF7 cells labeling CacyBP with ab171972 at 1/100 dilution.  DAPI nuclear staining (blue).

  • Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (ab171972)
    Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (ab171972)

    Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling CacyBP with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control.  HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

  • Flow Cytometry - Anti-CacyBP antibody [EPR12374] (ab171972)
    Flow Cytometry - Anti-CacyBP antibody [EPR12374] (ab171972)

    Flow cytometric analysis of permeabilized 293T cells labeling CacyBP with ab171972 at 1/10 dilution (red) or  Rabbit IgG (negative) (green).

  • Anti-CacyBP antibody [EPR12374] (ab171972)
    Anti-CacyBP antibody [EPR12374] (ab171972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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