Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CacyBP antibody [EPR12374] (ab171972)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12374] to CacyBP
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-CacyBP antibody [EPR12374]
See all CacyBP primary antibodies
Description

Rabbit monoclonal [EPR12374] to CacyBP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

within Human CacyBP aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HB71
Positive control
- Human kidney, gastric carcinoma and gliocytoma tissues; MCF7, HeLa, 293T and PC3 cell lysates; MCF7 and 293T cells.
General notes
This product was previously labelled as SIAH Interacting Protein

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12374
Isotype

IgG
Research areas

Western blot - Anti-CacyBP antibody [EPR12374] (ab171972)

All lanes : Anti-CacyBP antibody [EPR12374] (ab171972) at 1/10000 dilution

Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : PC3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)

Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling CacyBP with ab171972 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)

Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CacyBP antibody [EPR12374] (ab171972)

Immunofluorescent analysis of MCF7 cells labeling CacyBP with ab171972 at 1/100 dilution. DAPI nuclear staining (blue).
Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (ab171972)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling CacyBP with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Flow Cytometry - Anti-CacyBP antibody [EPR12374] (ab171972)

Flow cytometric analysis of permeabilized 293T cells labeling CacyBP with ab171972 at 1/10 dilution (red) or Rabbit IgG (negative) (green).
Anti-CacyBP antibody [EPR12374] (ab171972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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