Anti-CA19-9 antibody [121SLE] (ab3982)
![Anti-CA19-9 antibody [121SLE] (ab3982)](https://www.abcam.com/ps/products/3/ab3982/Images/ab3982-8456-anti-ca19-9-antibody-121sle-immunohistochemistry.jpg "Anti-CA19-9 antibody [121SLE] (ab3982)")
Key features and details
- Mouse monoclonal [121SLE] to CA19-9
- Suitable for: IHC-P, ELISA, Flow Cyt
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-CA19-9 antibody [121SLE]
See all CA19-9 primary antibodies -
Description
Mouse monoclonal [121SLE] to CA19-9 -
Host species
Mouse -
Specificity
The antibody recognizes Sialyl Lewis a containing glycolipids. -
Tested applications
Suitable for: IHC-P, ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CA19-9. Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.
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Positive control
- IHC-P: Colorectal adenocarcinoma.
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General notes
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 8.0
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
121SLE -
Isotype
IgM -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA19-9 antibody [121SLE] (ab3982)
ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).
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![Western blot - Anti-CA19-9 antibody [121SLE] (ab3982)](https://www.abcam.com/ps/products/3/ab3982/Images/ab3982-8455-anti-ca19-9-antibody-121sle-western-blot.jpg)
Western blot - Anti-CA19-9 antibody [121SLE] (ab3982)All lanes : Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml
Lane 2 : Human breast tissue lysate - total protein (ab30090)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
