Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CA19-9 antibody [121SLE] (ab3982)

Key features and details

  • Mouse monoclonal [121SLE] to CA19-9
  • Suitable for: IHC-P, ELISA, Flow Cyt
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-CA19-9 antibody [121SLE]
    See all CA19-9 primary antibodies

  • Description

    Mouse monoclonal [121SLE] to CA19-9

  • Host species

    Mouse

  • Specificity

    The antibody recognizes Sialyl Lewis a containing glycolipids.

  • Tested applications

    Suitable for: IHC-P, ELISA, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to CA19-9. Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.

  • Positive control

    • IHC-P: Colorectal adenocarcinoma.

  • General notes

    This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA19-9 antibody [121SLE] (ab3982)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA19-9 antibody [121SLE] (ab3982)

    ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).

  • Western blot - Anti-CA19-9 antibody [121SLE] (ab3982)
    Western blot - Anti-CA19-9 antibody [121SLE] (ab3982)
    All lanes : Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml

    Lane 2 : Human breast tissue lysate - total protein (ab30090)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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