Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C8orf30A antibody [EPR13203-28] (ab181031)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13203-28] to C8orf30A
  • Suitable for: IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-C8orf30A antibody [EPR13203-28]
    See all C8orf30A primary antibodies

  • Description

    Rabbit monoclonal [EPR13203-28] to C8orf30A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, Human spleen, HeLa and Caco2 lysates; Human breast carcinoma and Human endometrium tissues; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13203-28

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C8orf30A antibody [EPR13203-28] (ab181031)
    Western blot - Anti-C8orf30A antibody [EPR13203-28] (ab181031)
    All lanes : Anti-C8orf30A antibody [EPR13203-28] (ab181031) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human spleen lysate
    Lane 3 : HeLa lysate
    Lane 4 : Caco2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] (ab181031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] (ab181031)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrium tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] (ab181031)
    Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] (ab181031)

    Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (+) or a negative control (-) immunoprecipitated using ab181031 at 1/40 dilution.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-C8orf30A antibody [EPR13203-28] (ab181031)
    Immunocytochemistry/ Immunofluorescence - Anti-C8orf30A antibody [EPR13203-28] (ab181031)

    Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling C8orf30A with ab181031 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] (ab181031)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] (ab181031)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-C8orf30A antibody [EPR13203-28] (ab181031)
    Anti-C8orf30A antibody [EPR13203-28] (ab181031)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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