Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free
See all C4 binding protein/C4BPB primary antibodies
Description

Rabbit monoclonal [EPR17101] to C4 binding protein/C4BPB - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC, WB, IP, IHC-Pmore details
Species reactivity

Reacts with: Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab251274 is the carrier-free version of ab199430 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251274 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as C4 binding protein
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR17101
Isotype

IgG
Research areas

Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)

All lanes : Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/1000 dilution

Lane 1 : Human plasma
Lane 2 : Human ovary cancer extract.

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID: 2300577).
Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)

Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/10000 dilution + Human serum at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 28 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID: 2300577).
Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)

Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/1000 dilution + Rat plasma at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID: 2300577).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Plasma staining on Human bladder carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling C4 binding protein/C4BPB with ab199430 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab199430 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)

This data was developed using ab199430, the same antibody clone in a different buffer formulation.

C4 binding protein/C4BPB was immunoprecipitated from 1mg of Human serum with ab199430 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab199430 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human serum 10 µg. Lane 2: Human serum following immunoprecipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199430 in Human serum.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-C4 binding protein/C4BPB antibody [EPR17101] - BSA and Azide free (ab251274)