Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13213] to C21orf33 - BSA and Azide free
  • Suitable for: ICC, WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-C21orf33 antibody [EPR13213] - BSA and Azide free
    See all C21orf33 primary antibodies

  • Description

    Rabbit monoclonal [EPR13213] to C21orf33 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human C21orf33. The exact sequence is proprietary.
    Database link: P30042

  • General notes

    Ab250492 is the carrier-free version of ab181366. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250492 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    Immunoprecipitation - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    This data was developed using ab181366, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or 1XPBS (lane 2) labeling C21orf33 using ab181366 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Immunocytochemistry - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    Immunocytochemistry - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    This data was developed using ab181366, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HepG2 cells labeling C21orf33 using ab181366 at a 1/100 dilution (red) with DAPI nuclear staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    This data was developed using ab181366, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C21orf33 using ab181366 at a 1/50 dilution Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    This data was developed using ab181366, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C21orf33 using ab181366 at a 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Western blot - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    Western blot - Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    All lanes : Anti-C21orf33 antibody [EPR13213] (ab181366) at 1/5000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 25 kDa



    This data was developed using ab181366, the same antibody clone in a different buffer formulation.

     

  • Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)
    Anti-C21orf33 antibody [EPR13213] - BSA and Azide free (ab250492)

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