Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16094] to C10orf46/CAC1
  • Suitable for: WB, IHC-P, IP, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-C10orf46/CAC1 antibody [EPR16094]
    See all C10orf46/CAC1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16094] to C10orf46/CAC1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A375, HT1080, SW480 and Human breast cancer lysates; Human cervix squamous cell carcinoma and rat brain tissues; HeLa and A375 Cells.

  • General notes

     

     

     This product was previously labelled as C10orf46

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)
    Western blot - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)
    All lanes : Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : HT1080 cell lysate
    Lane 3 : SW480 cell lysate
    Lane 4 : Human breast cancer lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)
    Immunocytochemistry/ Immunofluorescence - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A375 cells labeling C10orf46/CAC1 with ab190799 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)
    Immunocytochemistry/ Immunofluorescence - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling C10orf46/CAC1 with ab190799 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue. Negative controls also shown.

  • Immunoprecipitation - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)
    Immunoprecipitation - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)

    Western blot analysis of C10orf46/CAC1 in immunoprecipitation pellets from HT1080 lysate. ab190799 used at 1/40 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling C10orf46/CAC1 with ab190799 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)

    Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling C10orf46/CAC1 with ab190799 at 1/50 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)
    Anti-C10orf46/CAC1 antibody [EPR16094] (ab190799)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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