Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-c-Kit antibody [017] - BSA and Azide free (ab276075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [017] to c-Kit - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-c-Kit antibody [017] - BSA and Azide free
    See all c-Kit primary antibodies

  • Description

    Rabbit monoclonal [017] to c-Kit - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human c-Kit aa 1-523 (extracellular).
    Database link: P05532

  • Positive control

    • WB: MOLT-4, Jurkat and K562 whole cell lysate. IP: K562 whole cell lysate.

Images

  • Western blot - Anti-c-Kit antibody [017] (ab276075)
    Western blot - Anti-c-Kit antibody [017] (ab276075)
    All lanes : Anti-c-Kit antibody [017] - BSA and Azide free (ab276075) at 1/500 dilution

    Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 109 kDa



    Developed using the Odyssey technique.

  • Immunoprecipitation - Anti-c-Kit antibody [017] (ab276075)
    Immunoprecipitation - Anti-c-Kit antibody [017] (ab276075)

    KIT was immunoprecipitated from 0.5 mg K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with 1 µL ab276075 and 15 µl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276075 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

    Developed using the odssey technique.
    Performed under reducing conditions.

  • Anti-c-Kit antibody [017] - BSA and Azide free (ab276075)
    Anti-c-Kit antibody [017] - BSA and Azide free (ab276075)

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