Anti-c-IAP1 and c-IAP2 antibody (ab25939)
Key features and details
- Rabbit polyclonal to c-IAP1 and c-IAP2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-c-IAP1 and c-IAP2 antibody -
Description
Rabbit polyclonal to c-IAP1 and c-IAP2 -
Host species
Rabbit -
Specificity
This antibody detects both c-IAP1 and c-IAP2. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to 14 C terminal amino acids of Human cIAP1
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Positive control
- A549 whole cell lysate (ab7910) can be used as a positive control in WB. Human lung cell lysate can be used as positive control.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
cIAP Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)All lanes : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 1 : Human Thymus
Lane 2 : Human Lung
Lane 3 : Human Testis
Lane 4 : Human Small Intestine
Lane 5 : Rat Brain
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)All lanes : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 1 : 293 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : A549 cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml
All lanes : human lung lysate
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Western blot - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Lane 1 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 1 µg/ml
Lane 2 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 2 µg/ml
Lane 3 : Anti-c-IAP1 and c-IAP2 antibody (ab25939) at 4 µg/ml
All lanes : Human lung lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-IAP1 and c-IAP2 antibody (ab25939)ab25939 at 10µg/ml staining cIAP1 in human lung cells by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)ICC/IF image of ab25939 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-c-IAP1 and c-IAP2 antibody (ab25939)Immunofluorescence of cIAP in Human Lung cells using ab25939 at 20 ug/ml.