Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20652] to BubR1 - BSA and Azide free
  • Suitable for: WB, IP, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-BubR1 antibody [EPR20652] - BSA and Azide free
    See all BubR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20652] to BubR1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251514 is the carrier-free version of ab209998. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251514 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)

    This data was developed using ab209998, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling BubR1 with ab209998 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific staining on centromeres of HT-29 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Immunoprecipitation - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    Immunoprecipitation - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)

    This data was developed using ab209998, the same antibody clone in a different buffer formulation.

    BubR1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab209998 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209998 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input).

    Lane 2: ab209998 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209998 in HeLa whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 1 second.



    All lanes : Anti-BubR1 antibody [EPR20652] (ab209998) at 1/1000 dilution

    All lanes :

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366)

    Observed band size: 120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second
  • Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    This data was developed using ab209998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling BubR1 with ab209998 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific staining on centromeres of A549 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    This data was developed using ab209998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling BubR1 with ab209998 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific staining on centromeres of HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
  • Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    This data was developed using ab209998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 with ab209998 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing subcellular localization of BubR1 changes through mitosis of HeLa cell line (PMID: 25833949). The nuclear counter stain is DAPI (blue).
  • Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    Immunocytochemistry - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    This data was developed using ab209998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 with ab209998 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific staining on centromeres of HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.
    -ve control: Secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.
  • Western blot - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    Western blot - Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    All lanes : Anti-BubR1 antibody [EPR20652] (ab209998) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Performed under non-reducing conditions.

    Predicted band size: 119 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    This data was developed using ab209998, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 3 seconds; Lane 3: 3 minutes.

    Positive BubR1 detection by western blot is dependent on the number of mitotic cells in the sample and may need optimization for some cell and tissue lysates.

  • Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)
    Anti-BubR1 antibody [EPR20652] - BSA and Azide free (ab251514)

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