Anti-BSD antibody (ab38307)
Key features and details
- Rabbit polyclonal to BSD
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-BSD antibody -
Description
Rabbit polyclonal to BSD -
Host species
Rabbit -
Specificity
The antibody detects a 13 kDa protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells. -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide conjugated to KLH corresponding to amino acid residues in the N-terminal region of Blasticidin S Deaminase from Aspergillus Terrus.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified using the N-terminal Bsd peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BSD antibody (ab38307)All lanes : Anti-BSD antibody (ab38307)
Lane 1 : 293 cells mock transfected
Lanes 2-3 : 293 cells transiently transfected with pLenti6/TR lentiviral vector
Observed band size: 13 kDa why is the actual band size different from the predicted?
Each lane was loaded with 10-20µg total protein. -
Immunocytochemistry/ Immunofluorescence - Anti-BSD antibody (ab38307) Image courtesy of Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Centerab38307, staining BSD in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were fixed in paraformaldehyde (3.75%) for 30 min., then permeabilized in 0.2% Triton X-100 for 15 min. Blocking was done using 1% BSA for 30 min. The primary was used at 1/100 and then an appropriate secondary antibody conjugated to Texas Red was used.
