Anti-Brn-2 antibody [CL6228] (ab243045)
![Anti-Brn-2 antibody [CL6228] (ab243045)](https://www.abcam.com/ps/products/243/ab243045/Images/ab243045-331778-anti-brn-2-antibody-cl6228-immunofluorescence.jpg "Anti-Brn-2 antibody [CL6228] (ab243045)")
Key features and details
- Mouse monoclonal [CL6228] to Brn-2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Brn-2 antibody [CL6228]
See all Brn-2 primary antibodies -
Description
Mouse monoclonal [CL6228] to Brn-2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseWB MouseHuman
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Immunogen
Recombinant fragment corresponding to Human Brn-2 aa 1-100.
Database link: P20265 -
Positive control
- WB: Mouse and human cerebral cortex tissue lysate; U-251 MG and HEK-293 cell lysate. IHC-P: Mouse cerebral cortex and forebrain tissue. ICC/IF: U-251 MG cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL6228 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [CL6228] (ab243045)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Brn-2 (green) using ab243045 at 10 µg/ml in ICC/IF. Microtubules are stained red.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)](https://www.abcam.com/ps/products/243/ab243045/Images/ab243045-331777-anti-brn-2-antibody-cl6228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)Paraffin-embedded mouse forebrain tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis.
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![Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)](https://www.abcam.com/ps/products/243/ab243045/Images/ab243045-451627-AntiBrn2-antibody-CL6228-western-blot-hek293-sirna-human.jpg)
Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)All lanes : Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cells transfected with control siRNA
Lane 2 : HE-K293 cells transfected with target specific siRNA probe #1
Predicted band size: 47 kDaRemaining relative intensity is presented.
Loading control: Anti-GAPDH
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![Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)](https://www.abcam.com/ps/products/243/ab243045/Images/ab243045-331773-anti-brn-2-antibody-cl6228-western-blot.jpg)
Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 47 kDa
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![Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)](https://www.abcam.com/ps/products/243/ab243045/Images/ab243045-331774-anti-brn-2-antibody-cl6228-western-blot.jpg)
Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/ml + Mouse cerebral cortex tissue lysate
Predicted band size: 47 kDa
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![Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)](https://www.abcam.com/ps/products/243/ab243045/Images/ab243045-331772-anti-brn-2-antibody-cl6228-western-blot.jpg)
Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/ml + Human cerebral cortex tissue lysate
Predicted band size: 47 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)](https://www.abcam.com/ps/products/243/ab243045/Images/ab243045-331775-anti-brn-2-antibody-cl6228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)Paraffin-embedded mouse cerebral cortex tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis. Reactivity observed in layer 2-4 neurones.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)](https://www.abcam.com/ps/products/243/ab243045/Images/ab243045-331776-anti-brn-2-antibody-cl6228-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)Paraffin-embedded mouse cerebral cortex tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis. No reactivity in layer 6 neurones.
