Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23926-112] to Bestrophin/BEST1
  • Suitable for: ICC, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Bestrophin/BEST1 antibody [EPR23926-112]
    See all Bestrophin/BEST1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23926-112] to Bestrophin/BEST1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IPmore details
    Unsuitable for: Flow Cyt,IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis and lung tissue lysate. Human eyeball tissue lysate. Y79 whole cell lysate. ICC: Y79 cells. IP: Y79 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23926-112

  • Isotype

    IgG

Images

  • Western blot - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    Western blot - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    All lanes : Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 68 kDa



    Low expression: Human liver(PMID:25941382).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lane 2-3: 3 minutes.

  • Immunocytochemistry - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    Immunocytochemistry - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Y79 (human retinoblastoma retinoblastoma) cells labeling Bestrophin/BEST1 with ab259836 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Y79 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    Immunoprecipitation - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)

    Bestrophin/BEST1 was immunoprecipitated from 10 µg of Y79 (human retinoblastoma retinoblastoma) whole cell lysate with ab259836 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed from the immunoprecipitate using ab259836 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Input: 10 µg of Y79 whole cell lysate.
    Lane 2: Y79 whole cell lysate following IP with ab259836.
    Lane 3: Negative control: IP using Rabbit monoclonal IgG (ab172730) instead of ab259836 in Y79 whole cell lysates.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

  • Western blot - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    Western blot - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836) at 1/1000 dilution + human eyeball tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 68 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    Western blot - Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836) at 1/1000 dilution + Y79 (human retinoblastoma retinoblastoma), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)
    Anti-Bestrophin/BEST1 antibody [EPR23926-112] (ab259836)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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