Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [008] to BDP / ARID3B - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-BDP / ARID3B antibody [008] - BSA and Azide free
    See all BDP / ARID3B primary antibodies

  • Description

    Rabbit monoclonal [008] to BDP / ARID3B - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human BDP/ ARID3B.
    Database link: Q8IVW6

  • Positive control

    • IP: K562 and HEK-293T cells. WB: K562, HEK-293T and HepG2 cells.

Properties

Images

  • Immunoprecipitation - Anti-BDP / ARID3B antibody [008] (ab276048)
    Immunoprecipitation - Anti-BDP / ARID3B antibody [008] (ab276048)

    ARID3B was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia lymphoblast cell line ) (lane A) and HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (lane B) whole cell lysate with 2 µL ab276048 and 15 µl of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab276048 at 1/100 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibodyat 1/5000 dilution.

    Developed using the odssey technique.
    Performed under reducing conditions.

  • Western blot - Anti-BDP / ARID3B antibody [008] (ab276048)
    Western blot - Anti-BDP / ARID3B antibody [008] (ab276048)
    All lanes : Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 61 kDa



    Developed using the Odyssey technique.

    Performed under reducing conditions.

  • Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)
    Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)

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