Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)
![Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)](https://www.abcam.com/ps/products/276/ab276048/Images/ab276048-2-AntiBDP-ARID3B-antibody-008-IP-K562-293T-cells.jpg "Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [008] to BDP / ARID3B - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
-
Product name
Anti-BDP / ARID3B antibody [008] - BSA and Azide free
See all BDP / ARID3B primary antibodies -
Description
Rabbit monoclonal [008] to BDP / ARID3B - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human BDP/ ARID3B.
Database link: Q8IVW6 -
Positive control
- IP: K562 and HEK-293T cells. WB: K562, HEK-293T and HepG2 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
008 -
Isotype
IgG -
Research areas
Images
-
Immunoprecipitation - Anti-BDP / ARID3B antibody [008] (ab276048)
ARID3B was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia lymphoblast cell line ) (lane A) and HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (lane B) whole cell lysate with 2 µL ab276048 and 15 µl of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab276048 at 1/100 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibodyat 1/5000 dilution.
Developed using the odssey technique.
Performed under reducing conditions. -
![Western blot - Anti-BDP / ARID3B antibody [008] (ab276048)](https://www.abcam.com/ps/products/276/ab276048/Images/ab276048-1-AntiBDP-ARID3B-antibody-008-wb-293T-K562-HepG2-cells.jpg)
Western blot - Anti-BDP / ARID3B antibody [008] (ab276048)All lanes : Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 61 kDaDeveloped using the Odyssey technique.
Performed under reducing conditions.
-
![Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)](https://www.abcam.com/ps/products/276/ab276048/Images/ab276048-3-benefits-of-recombinant-antibodies.png)
Anti-BDP / ARID3B antibody [008] - BSA and Azide free (ab276048)
