Anti-Bcr-abl antibody [7C6] - BSA and Azide free (ab256126)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [7C6] to Bcr-abl - BSA and Azide free
- Suitable for: WB, ICC
- Reacts with: Human
Overview
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Product name
Anti-Bcr-abl antibody [7C6] - BSA and Azide free
See all Bcr-abl primary antibodies -
Description
Mouse monoclonal [7C6] to Bcr-abl - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 whole cell lysate. ICC: K562 cells.
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General notes
ab256126 is the carrier-free version of ab187831. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256126 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7C6 -
Isotype
IgG2a
Images
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This data was developed using ab187831, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 fixed K562 (human chronic myelogenous leukemia lymphoblast) cells labeling Bcr-abl with ab187831 at 1/250 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary antibody at 1/1000 dilution (Green). Confocal image showing strongly cytoplasmic staining in K-562 cell line. The nuclear counter stain id DAPI (Blue). Tubulin is detected using ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red).
The negative controls are as follows:
-ve control 1: ab187831 at 1/250 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) secondary at 1/500 dilution.
-ve control 2: Primary (ab179513) at 1/200 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1/1000 dilution. -
All lanes : Anti-Bcr-abl antibody [7C6] (ab187831) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 197 kDa
Observed band size: 142,210 kDa why is the actual band size different from the predicted?This data was developed using ab187831, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates should be non-boiled as boiling may cause protein aggregates.
Exposure time: 59 seconds
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