Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP10625] to Bcl2-L-13 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free
    See all Bcl2-L-13 primary antibodies

  • Description

    Rabbit monoclonal [EP10625] to Bcl2-L-13 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251397 is the carrier-free version of ab203516. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251397 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EP10625

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    Western blot - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    Anti-Bcl2-L-13 antibody [EP10625] (ab203516) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab203516, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    Western blot - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    All lanes : Anti-Bcl2-L-13 antibody [EP10625] (ab203516) at 1/5000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab203516, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    This data was developed using ab203516, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on human pancreas tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    This data was developed using ab203516, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on human tonsil tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    This data was developed using ab203516, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on mouse cardiac muscle tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    This data was developed using ab203516, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Bcl2-L-13 with ab203516 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining on rat kidney tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)
    Anti-Bcl2-L-13 antibody [EP10625] - BSA and Azide free (ab251397)

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