Anti-BCA1 antibody (ab272874)
Key features and details
- Rabbit polyclonal to BCA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BCA1 antibody
See all BCA1 primary antibodies -
Description
Rabbit polyclonal to BCA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BCA1 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: O43927 -
Positive control
- IHC-P: Human esophageal carcinoma tissue. ICC/IF: HepG2 cells. WB: Transfected 293T whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody (ab272874)
Paraffin-embedded human esophageal carcinoma tissue stained for BCA1 using ab272874 at a 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-BCA1 antibody (ab272874)4% PFA-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for BCA1 using ab272874 at 1/500 dilution in ICC/IF (green).
Blue: Hoechst 33342 staining.
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Western blot - Anti-BCA1 antibody (ab272874)All lanes : Anti-BCA1 antibody (ab272874) at 1/5000 dilution
Lane 1 : Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected (+) HEK-293T whole cell lysate
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCA1 antibody (ab272874)Paraffin-embedded human esophageal carcinoma tissue stained for BCA1 using ab272874 at a 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
