Anti-B7H4 antibody [7A1-6] (ab281918)
![Anti-B7H4 antibody [7A1-6] (ab281918)](https://www.abcam.com/ps/products/281/ab281918/Images/ab281918-1-AntiB7H4-antibody-immunohistochemistry-RAW2647-cells.jpg "Anti-B7H4 antibody [7A1-6] (ab281918)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7A1-6] to B7H4
- Suitable for: ICC
- Reacts with: Mouse
Overview
-
Product name
Anti-B7H4 antibody [7A1-6]
See all B7H4 primary antibodies -
Description
Rat monoclonal [7A1-6] to B7H4 -
Host species
Rat -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to B7H4. Raised by immunising rats with the recombinant murine B7-H4.
Database link: Q7TSP5 -
Positive control
- ICC: RAW264.7 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7A1-6 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
-
Immunocytochemistry - Anti-B7H4 antibody [7A1-6] (ab281918)
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells stained with the chimeric rabbit IgG version of this antibody at 10 µg/ml for 1hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom chimeric antibody, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. This data was developed using the chimeric version.