HEK-293 (human epithelial cell line from embryonic kidney) cells were stably transfected with BR2. Cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45°C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with ab277806 at a 1/500 dilution.

Lane 1: Mock transfected.

Lane 2: B2R transfected.

Sections of human pancreas were dewaxed, microwaved in citric acid and incubated with ab277806 at a 1/500 dilution. Sections were then sequentially treated with biotinylated Anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Control inset.