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Anti-AZI1 antibody (ab99315)

Anti-AZI1 antibody (ab99315)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to AZI1
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-AZI1 antibody
    See all AZI1 primary antibodies
  • Description

    Rabbit polyclonal to AZI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Immunogen corresponds to a region between residues 1033 and 1083 of Human AZI1 (EAW89632.1)

  • Positive control

    • Hela whole cell lysate and 293T cell lysate
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

Images

  • Western blot - Anti-AZI1 antibody (ab99315)
    Western blot - Anti-AZI1 antibody (ab99315)
    All lanes : Anti-AZI1 antibody (ab99315) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 122 kDa


    Exposure time: 10 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99315)
    Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99315)
    Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99315 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • Immunoprecipitation - Anti-AZI1 antibody (ab99315)
    Immunoprecipitation - Anti-AZI1 antibody (ab99315)
    ab99315 at 1 µg/ml detecting AZI1 in HeLa whole cell lysate by WB following IP.
    Lane 1: IP with an antibody which recognizes an upstream epitope of AZI1
    Lane 2: IP with an another antibody which recognizes an upstream epitope of AZI1
    Lane 3: ab99315 at 3µg/mg of lysate
    Lane 4: control IgG.
    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
    Detection: Chemiluminescence with an exposure time of 3 seconds

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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