Anti-AZI1 antibody (ab99315)
Key features and details
- Rabbit polyclonal to AZI1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-AZI1 antibody
See all AZI1 primary antibodies -
Description
Rabbit polyclonal to AZI1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Immunogen corresponds to a region between residues 1033 and 1083 of Human AZI1 (EAW89632.1)
-
Positive control
- Hela whole cell lysate and 293T cell lysate
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-AZI1 antibody (ab99315) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 10 seconds
-
Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99315 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
-
ab99315 at 1 µg/ml detecting AZI1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of AZI1
Lane 2: IP with an another antibody which recognizes an upstream epitope of AZI1
Lane 3: ab99315 at 3µg/mg of lysate
Lane 4: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds