Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [7E10] to Axl - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Axl antibody [7E10] - BSA and Azide free
    See all Axl primary antibodies

  • Description

    Mouse monoclonal [7E10] to Axl - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HeLa cells. ICC/IF: HeLa cells. WB: DU145, NCI-H1299 and HeLa whole cell lysates.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264492 is the carrier-free version of ab130218. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)
    Western blot - Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)
    All lanes : Anti-Axl antibody [7E10] (ab130218) at 1.947 µg/ml

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : DU145 (human prostate carcinoma epithelial cell), whole cell lysate
    Lane 3 : NCI-H1299 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 98 kDa
    Observed band size: 140,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    The molecular weight observed was in consistant with the literature (PMID: 19541935).

    Negative control: Jurkat (PMID: 28423548).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab130218).

  • Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)
    Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)

    Immunofluorescent analysis of 4% methanol-fixed, 0.1% Triton X-100 permeabilized NCI-H1299 cells labeling Axl with ab130218 at 7.788 µg/mL, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on NCI-H1299 cell line.

    Negative control: Jurkat (PMID:28423548).

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab130218 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179513 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab130218).

  • Flow Cytometry - Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)
    Flow Cytometry - Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte, Left panel) / HeLa (Human cervix adenocarcinoma epithelial cell, Right panel) labeling Axl using Anti-Axl antibody [7E10] (ab130218) at a 0.974 µg/mL (Red) followed by Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at a 1/2000. Mouse monoclonal IgG isotype control (Black). The unlabeled control is cells without incubation with primary antibody or secondary antibody (Blue).

    Negative control: Jurkat.

    Gated on viable cells.

    This image was produced using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab130218).

  • Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)
    Anti-Axl antibody [7E10] - BSA and Azide free (ab264492)

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