Anti-ATP5A antibody (ab188107)
Key features and details
- Goat polyclonal to ATP5A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-ATP5A antibody
See all ATP5A primary antibodies -
Description
Goat polyclonal to ATP5A -
Host species
Goat -
Specificity
ab188107 is expected to recognize all reported isoforms (NP_004037.1; NP_001244263.1; NP_001001935.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cow, Dog, Xenopus laevis, Chimpanzee, Orangutan
-
Immunogen
Synthetic peptide corresponding to Human ATP5A aa 50-150 (internal sequence) (Cysteine residue). (NP_004037.1).
Database link: P25705 -
Positive control
- WB: Human, mouse fetal, mouse, pig and rat heart lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffer, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab188107 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ATP5A antibody (ab188107)All lanes : Anti-ATP5A antibody (ab188107) at 0.01 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Fetal mouse heart lysate (in RIPA buffer)
Lane 3 : Adult mouse heart lysate (in RIPA buffer)
Lane 4 : Adult rat heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 60, 54 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
-
Western blot - Anti-ATP5A antibody (ab188107)Anti-ATP5A antibody (ab188107) at 0.01 µg/ml + Pig heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 60, 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
