Anti-ATP1B3 antibody [EPR8981] (ab137055)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8981] to ATP1B3
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ATP1B3 antibody [EPR8981]
See all ATP1B3 primary antibodies -
Description
Rabbit monoclonal [EPR8981] to ATP1B3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide corresponding to amino acids near the C terminal of Human ATP1B3 (UniProt ID: P54709).
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Positive control
- A431 and HeLa cell lysates; Human cervical squamous carcinoma and Human liver tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8981 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labelling ATP1B3 with ab137055 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ATP1B3 with ab137055 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ATP1B3 antibody [EPR8981] (ab137055) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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