Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Wednesday March 10, 2021

Anti-ATP1B3 antibody [EPR8981] (ab137055)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8981] to ATP1B3
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-ATP1B3 antibody [EPR8981]
See all ATP1B3 primary antibodies
Description

Rabbit monoclonal [EPR8981] to ATP1B3
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to amino acids near the C terminal of Human ATP1B3 (UniProt ID: P54709).
Positive control
- A431 and HeLa cell lysates; Human cervical squamous carcinoma and Human liver tissues
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8981
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)

Immunohistochemical analysis of paraffin-embedded Human cervical squamous carcinoma tissue labelling ATP1B3 with ab137055 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP1B3 antibody [EPR8981] (ab137055)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling ATP1B3 with ab137055 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-ATP1B3 antibody [EPR8981] (ab137055)

All lanes : Anti-ATP1B3 antibody [EPR8981] (ab137055) at 1/1000 dilution

Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 32 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Anti-ATP1B3 antibody [EPR8981] (ab137055)

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