Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13421] to ATHL1 - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ATHL1 antibody [EPR13421] - BSA and Azide free
    See all ATHL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13421] to ATHL1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251424 is the carrier-free version of ab205302. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251424 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    Western blot - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    All lanes : Anti-ATHL1 antibody [EPR13421] (ab205302) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate
    Lane 3 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 1 minute


    This data was developed using ab205302, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    Western blot - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    Anti-ATHL1 antibody [EPR13421] (ab205302) at 1/1000 dilution + Human fetal kidney tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 3 minutes


    This data was developed using ab205302, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    Immunocytochemistry - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    This data was developed using ab205302, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ATHL1 with ab205302 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing cytoplasmic staining on MCF-7 cell line.The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab205302 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Immunocytochemistry - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    Immunocytochemistry - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    This data was developed using ab205302, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling ATHL1 with ab205302 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).Confocal image showing cytoplasmic staining on BxPC-3 cell line.The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:--ve control 1: ab205302 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)
    Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (ab251424)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.