Anti-Ataxin 7 antibody (ab11434)
Key features and details
- Rabbit polyclonal to Ataxin 7
- Suitable for: ICC/IF, WB
- Reacts with: Human, Drosophila melanogaster, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-Ataxin 7 antibody
See all Ataxin 7 primary antibodies -
Description
Rabbit polyclonal to Ataxin 7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Drosophila melanogaster, African green monkey
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human Ataxin 7 aa 1-17.
Sequence:MSERAADDVRGEPRRAA
(Peptide available asab39761)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ataxin 7 antibody (ab11434)Western blot of Ataxin 7 in transfected COS-1 cell lysate using ab11434.
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Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 7 antibody (ab11434)ICC/IF image of ab11434 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11434, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
