Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7220(2)] to ART3 - C-terminal
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ART3 antibody [EPR7220(2)] - C-terminal
    See all ART3 primary antibodies

  • Description

    Rabbit monoclonal [EPR7220(2)] to ART3 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human testis tissue; Human testis lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7220(2)

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ART3 using ab192870 at 1/500 dilution (0.6 μg/ml). A prediluted HRP Polymer for Rabbit IgG  was used as secondary. Counterstain: Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)
    Western blot - Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)
    Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870) at 1/10000 dilution + Human testis tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 41 kDa (possible mature (processed) protein)

  • Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)
    Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)

    Immunoprecipitation analysis of Human testis lysate labeling ART3 using ab192870 at 1/20 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody

    Note: The band observed at 41 kDa may be due to the removal of a 27 amino acid propeptide (mature form).

  • Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)
    Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.